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Book Stain, Periodic Acid-Schiff (PAS) Appointment Online at the best price in Delhi/NCR from Ganesh Diagnostic. NABL & NABH Accredited Diagnostic centre in Delhi offering a wide range of Radiology & Pathology tests. Get Free Ambulance & Free Sample collection from Home. 24 Hour Open.
The Periodic Acid-Schiff Dye is a widely utilized strategy in histopathology for the indication of glucose and glucose-rich mixtures in tissues. PAS dyes demonstrate polysaccharides, mucin, glycogen, specific glycoproteins and glycolipids, basement membrane, and specific fungus in tissues.
The Glycol class of carbohydrates is corroded by occasional acids to discharge dialdehydes. These dialdehydes in subsequent mixture with Schiff’s reagent outcome in the appearance of magenta colored intricate, localized at the area of aldehyde impression.
The occasional acid solution, utilized for oxidation is utilized in any resilience between 0.49% and 2.49%. One percent solution is selected.
>> Occasional acid= one gm
>> Distilled water= Hundred ml
>> Fuchsin Basic: 0.9 gm
>> Purified water: 100 ml
>> Sodium metabisulphite: 1.9 gm
>> Conc. HCl: 1.9 ml
>> Charcoal activated: 0.29 gm
The substances favorable for PAS are
Polysaccharides: Glycogen, cellulose, and starch. Many leukocytes comprise glycogen, capsules of fungi, actinomycosis, and bacteria.
| Test Type | Stain, Periodic Acid-Schiff (PAS) |
| Includes | Stain, Periodic Acid-Schiff (PAS) Test (Hematology) |
| Preparation | |
| Reporting | Within 24 hours* |
| Test Price |
₹ 400
|
It is seen that periodic acid-Schiff (PAS) staining procedure could be considered most commonly being used in your histology laboratory to further detect your glycogen deposits in liver. This is where the glycogen storage disease could be suspected. Moreover, the Glycogen granules would be visible in the tumors of the following:
In general, it is seen that the glycogen could be digested by the enzyme diastase whereas your neutral mucosubstances cannot do the same. Hence, your glycogen shows the PAS-positive whereas PASD-negative staining pattern while the neutral mucosubstances could have the double positive pattern.
The PAS staining (Periodic Acid-Schiff) is said to be one of the most commonly used stains in the histology department and it is also used to further evidence presence of an aldehyde groups which gets formed by your prior test with oxidation of any carbohydrates.
Your Good Schiff's reagent could have ability to the rapidly turn into a red purple color. When there is a deteriorating with Schiff's reagent will with any delayed reaction with color produced would be a deep blue-purple.
The drawback and limitation of the PAS stain with your comparison to any GMS is which would not visualize any of the fungal species well, and the non-viable organisms which usually are not that well stained.
For your PAS (Periodic Acid-Schiff): The Harris Hematoxylin Histology Stain is used.
When it is designed for only the research use, it would not be approved for the human or the animal use, along with clinical diagnosis.
You could get yourself tested for Periodic Acid-Schiff (PAS) at Ganesh Diagnostic and Imaging Center. We would be happy to assist you in getting your sample by trained staff and technicians.
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