Methotrexate Drug Level Test and Its Cost

Methotrexate Drug Level

Book Methotrexate Drug Level Test at the best price in Delhi NCR, India from GDIC (Ganesh Diagnostic & Imaging Centre). GDIC is a NABL Accredited Pathology lab in Delhi offering a wide range of lab testing services. What is Methotrexate Drug Level Test? Methotrexate is a...

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Book Methotrexate Drug Level Test at the best price in Delhi NCR, India from GDIC (Ganesh Diagnostic & Imaging Centre). GDIC is a NABL Accredited Pathology lab in Delhi offering a wide range of lab testing services.

What is Methotrexate Drug Level Test?

Methotrexate is a medication used to treat childhood acute lymphocytic leukaemia, lymphoma, lung, head, neck, and breast cancers, as well as rheumatoid arthritis and psoriasis.

This test aids in identifying and assessing methotrexate-toxic amounts.

When Will I Be Tested?

When symptoms signal methotrexate toxicity, at predetermined timed intervals following a high dosage of methotrexate to monitor blood concentrations and direct treatment.

Describe methotrexate.

Depending on the patient's risk factors, methotrexate can be used alone or in combination regimens to treat choriocarcinoma and associated trophoblastic tumours in female patients. Most patients receiving low doses of methotrexate and leucovorin have claimed to heal

Although methotrexate promotes full remission in juvenile acute lymphocytic leukaemia, it is more useful for maintenance therapy and is the preferred drug when combined with mercaptopurine. Furthermore, patients with acute lymphocytic leukaemia typically get intrathecal methotrexate and cranial irradiation.

Breast, lung, bladder, cervical, gastric, and ovarian carcinomas as well as non-Hodgkin and Burkitt lymphomas are all treated with combination regimens that include methotrexate. Osteogenic sarcoma, a few non-Hodgkin lymphomas, and (according to some doctors) head and neck tumours are all treated with high-dose methotrexate + leucovorin rescue (with or without additional medications).

The main medication used to treat medulloblastoma and cutaneous T-cell lymphomas is methotrexate. It has demonstrated activity as a single agent against testicular cancer, soft tissue sarcomas, embryonal rhabdomyosarcoma, bladder, lung, colorectal, oesophagal, and cervical carcinomas. More efficient regimens are available and used significantly more commonly than methotrexate for certain of these neoplasms (testicular, colorectal, and liver cancers; soft tissue sarcomas and rhabdomyosarcoma).

Why is it done?

This is used to determine whether methotrexate is working and causing any side effects. Depending on the results of your tests, your doctor may increase or decrease your methotrexate dose.

What is under examination?

The quantity of methotrexate in the blood is determined by this test. Since the 1960s, methotrexate has been a common medication. Acute lymphocytic leukaemia in children and certain lymphomas are the main conditions it is used to treat. It is used to treat rheumatoid arthritis (RA), severe Crohn's disease, psoriasis, non-Hodgkin lymphoma, and breast, head, neck, and lung malignancies in people. The folate vitamin is affected by methotrexate. The immune system's activity is reduced and cancer cell development is slowed by this action. In addition to acting as an anti-inflammatory, methotrexate helps lessen the joint damage brought on by RA.

Methotrexate needs to be closely watched. It has serious negative effects even when used correctly. Increased quantities can be toxic, perhaps harming the liver, kidneys, and lungs as well as inhibiting bone marrow's ability to produce new cells.

The ailment being treated determines the dosage of methotrexate be administered. For non-cancer illnesses, low-dose treatment is typically administered once a week, with levels in the blood rising after a dose and then gradually declining. Since the kidneys are responsible for removing methotrexate from the body, any condition that impairs kidney function or interferes with medication excretion may cause blood concentrations to rise.

How does one prepare for the test? 

No preparation is needed.

What is the procedure for Methotrexate Drug Test?

You'll have a check-up and blood tests every 1 to 2 weeks when you first start treatment.

  • Following the administration of high-dose methotrexate, a methotrexate test is routinely required at 24, 48, and 72 hours, depending on the situation.
  • Throughout methotrexate treatment, tests to check kidney and liver function as well as blood cell counts are also required regularly.

Required Documents for

  • A doctor's prescription. 
  • A valid government ID.


No care is required.

The cost of Methotrexate Drug Test in Delhi

The Methotrexate Drug Test costs vary by region and facility. So, get tested at trustworthy diagnostic centre like Ganesh Diagnostic. Training and experienced professionals are staffed to assure patient and visitor safety.

Test Type Methotrexate Drug Level

Methotrexate Drug Level Test (Microbiology)


Within 24 hours*

Test Price ₹ 1600 ₹ 3200
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