Epstein-Barr Virus DNA, Quantitative, Real-Time PCR - Test, Procedure & Cost

Epstein-Barr virus (EBV) is the causative vendor of infectious mononucleosis and is determined with posttransplant lymphoproliferative infection (PTLD), Hodgkin's lymphoma, Burkitt's lymphoma, nasopharyngeal carcinoma, and HIV-associated lymphomas.

Surveying EBV DNA grades by quantitative PCR in patients at the chance of EBV-associated lymphoproliferative diseases may permit punctual recognition of virus reactivation and access to the installment of antiviral therapy.

This assay is aimed at being utilized for the quantitative detection of Epstein-Barr virus (EBV) DNA and as a support in the diagnosis and surveillance of EBV illnesses. This assay is traceable to the 1st WHO International Standard for Epstein Barr Virus for Nucleic Acid Amplification Techniques (NAT)-based assays.

Sample Needed:

Plasma (EDTA)

Procedure

PCR amplification

Instructions:

Specimen Processing:

• Centrifuge for 20 minutes at 1600 x g within 4 hours of compilation.
• Aseptically go into the tube and aliquot plasma into sterile tubes.
• Stock at 2-8°C for 72 hours or -20 to -80°C extended terms.

Conveyance Instructions:

• Position coolant packs into styrofoam receptacles.
• Position the petition into the outside pocket of the pouch.
• Safeguard specimens by covering them in bubble wrap or toweling.
• Suggest express mail or counterpart if not on courier service.

Specimen Preparation

• Distinct serum or plasma from cells.
• Transmit 1 mL of serum, plasma, whole blood, or CSF to a clean container. (Min: 0.5 mL)

Storage/Transport Temperature

• Frosty.
• Whole Blood: Refrigerated.
• Illustrative Data:

Experimental spectrum value in log10 values:

• 2.7 - 8.48 IU/mL (500-300,000,000 IU/mL non-log converted significances)
• Favorable results slighter than 500 IU/mL will be noted as "POS <2.7 LOG IU" ("POS <500 IU/mL")
• Unfavorable results will be noted as "Not caught" (NOT DET).
• The method is calibrated to the WHO international criterion.