Direct Antiglobulin Test (DAT) with Reflex to Anti C3 and Anti IgG Cost & Procedure

To study a blood transfusion reaction, to identify the source of red blood cell destruction (hemolytic anaemia), and to identify infant hemolytic illness

When Will I Be Tested?

If you just received a blood transfusion and are now suffering transfusion responses symptoms,s including dizziness, back discomfort, dark urine, or shortness of breath

When a newborn exhibits symptoms of hemolytic illness,

When you experience symptoms of increased red blood cell destruction, such as fatigue more frequently than usual, shortness of breath, headaches, and pallor, your doctor may order tests to determine whether this is due to your immune system producing antibodies that accidentally destroy red blood cells.

Sample Necessary:

A blood sample is taken from an arm vein

What is under examination?

Your bloodstream's red blood cells (RBCs) are checked using the direct antiglobulin test (DAT) to see if they are covered in antibodies. The RBCs' demise is brought on by the antibodies that are adhered to their surface.

Many factors can lead to antibodies attacking RBCs, including:

• After receiving blood from a donor who does not comentirelytch their type, the recipient's body will produce antibodies that will mark the donor's RBC as foreign.
• These antibodies will target and kill the RBCs of the donor.
• Because they are exposed to more foreign RBCs, individuals who frequently receive blood transfusions are more prone to develop antibodies to RBCs.
• A DAT will be carried out if someone experiences response symptoms following a transfusion to see if the antibodies have bound to the RBCs.
• Mother/baby blood type incompatibility: A mother and baby may have different blood types if the baby inherits a blood type from the father.
• During pregnancy or lablabourhe mother
• maybe exposed to the baby’s RBCs.

What is the test used for?

• The direct antiglobulin test (DAT) is primarily used to detect whether antibodies bound to RBCs are the primary cause of hemolytic anaemia. Red blood cells (RBCs) are lost more quickly than they can be replaced in hemolytic anaemia, a disease.
• Due to an incompatibility between the mother's and the child's blood types, a DAT may also be performed to diagnose hemolytic disease of the newborn (HDN).
• It is also possible to utilise a DAT to determine a possible transfusion response.