The tri potassium phenolphthalein disulfate substrate is attacked by the arylsulfatase enzyme, which is produced by different Mycobacterium species. The medium turns red as a result of the reaction that releases phenolphthalein.

Why is this test suggested?

Our bodies contain an enzyme called arylsulfatase A, which is encoded by the ARSA gene and aids in the breakdown of sulfatides. The test aids in determining how much of the enzyme is present in body fluids. It aids in the identification of the genetic disease known as metachromatic leukodystrophy, which affects the muscles, nerves, and other organs. A blood sample, amniotic fluid, urine sample, or other bodily fluids can all be used to conduct the test.

Preparation for the Arylsulphatase A:

• Your doctor will also need your permission.
• Additionally, you must disclose to your doctor your medical history, current medications, and any recent operations.
• You simply need to confirm that you have acquired the necessary data.
• You could be requested to collect the first urine of the day for the urine test.
• Your doctor would inform you if anything additional was necessary.
• Your physician will provide you with advice based on your circumstances and state of health.

Arylsulphatase A Test Applications;

• The range of the test's normal results for 4-pyrocatechol is 32.0 to 129.6 nanomoles/ml of serum/4 hours.

Procedure:

• Your doctor will gather and analyze the material for the test.
• Your doctor or the lab technician will put a plastic band on your upper arm before totyoull the vein with blood and causing it to bulge.
• After using an antiseptic to disinfect the region, a needle will be inserted.
• The blood sample will then be collected and placed in a vial or tube.
• The needles are removed, and the region is then wrapped to halt the bleeding.