ANCA IIF Test Cost & It's Procedure

anca iif


Book ANCA IIF Appointment Online at the best price in Delhi/NCR from Ganesh Diagnostic. NABL & NABH Accredited Diagnostic centre in Delhi offering a wide range of Radiology & Pathology tests. Get Free Ambulance & Free Sample collection from Home. 24 Hour Open.

₹ 1460 ₹ 730

Request A Call Back

ANCA stands for Antineutrophil cytoplasmic antibodies test is used to measure the level of ANCA in blood.Antibodies are produced by our immune system to fight against the foreign antigens like Virus,Bacteria,parasitic infection invading your body.But sometimes our own immune cells starts to kill their own cells and leads to autoimmune disease.

ANCA attacks  white blood cells called neutrophils that play a very important role in countering the viral,pathogen and bacterial infection. Their proper cell count is also required for the proper functioning of our immune system to fight against diseases.

Due to this a condition called Autoimmune angiitis or vasculitis is arised that causes the inflammation and swelling of blood vessels due to this the blood vessels can narrow affecting the blood flow and this can cause the serious health condition if the test for ANCA is not done during early phase.

ANCA test checks for the presence of two types of antibodies called pANCA and cANCA each targets specific protein in the white blood cells by analysing the report helps to detect which type of angiitis you have and its diagnosis will help you to get the right treatment

Test for pANCA can be  used to diagnose the certain types of  Inflammatory bowel disease(IBD) that causes the swelling and irritation in the stomach and intestinal region

Procedure for ANCA IIF Test:

Indirect immunofluorescence assay(IIF) uses the ethanol fixed buffy coat that combined with the formalin fixed neutrophils and HEp-2 cells to discriminate between the P-ANCA and ANCA

It includes

  • The dilution of patient sera in 1/10 proportion to the three wells
  • If sera containing specific antibodies of IgG,IgAor IgM it got attached to antigen
  • Staining of Bound antibodies with green labelled anti human antibodies.
  • Observe under the fluorescence microscope
  • If the fluorescence is observed it shows that test is positive

Result of ANCA Test

If the granular fluorescence is observed  distributed regularly over entire cytoplasm it confirms cANCA

If  smooth fluorescence wrapped around the granulocytes it confirms pANCA

Use of ANCA Test

Helps in detecting Autoimmune vasculitis and its different types like:-

  1. Microscopic polyangiitis affects lung,liver, kidney and joints.
  2. Granulomatosis with polyangiitis-affects blood vessel of the lung and kidneys
  3. Eosinophilic granulomatosis with polyangiitis-  affects our nervous system,asthma,affects lungs,kidneys etc.
    • Detects crohn's disease and ulcerative colitis 
    • Monitoring the condition of Blood vessels affected
    • To check the neutrophils count and their number

Why is This Test Required?

If you have any symptoms of autoimmune angiitis or Inflammatory bowel diseases you may need to do this test common symptoms are

  • Fatigue
  • Loss of appetite
  • Weight loss
  • Aches and pains

Symptoms Affected With Any Particular Parts of the Body Includes:-

Eyes Ear Nose

  • Loss of vision
  • Hearing loss
  • Dizziness or lightheadedness
  • Sinus 


  • Heavy breathing
  • Blood in cough

Nervous System

  • Weakness of nerves 
  • numbness or tingling

Stomach and Intestines

  • Blood vomiting
  • diarrhoea

Symptoms of Inflammatory Bowel Disease Includes

Belly Pain

  • Blood in stool
  • Fever
  • Weight loss

Cost of ANCA IIF Test in Delhi

Cost of the ANCA IIF test in Delhi ranges from 3000-6000 Rs approx. You can check for the Best diagnosis centre, Ganesh diagnosis centre in Delhi provides you the best facility at the affordable price range of 3500 Rs. The test is conducted under the supervision of the best doctor and health practitioners.

Book Your Online Appointment at Ganesh Diagnostic Centre

At Ganesh diagnostic Centre you can book online appointment at your home with much affordable prices and get treated and improves your quality of life by scheduling your appointment at Ganesh diagnostics

Test Type ANCA IIF

ANCA IIF (Pathology Lab)


Within 24 hours*

Test Price ₹ 730 ₹ 1460
Frequently Asked Questions

It does not require any physical preparations and it may be done with other blood test

No, it only requires collection of blood samples so you may have slight pain at the spot where needle was pricked 

If the result are positive means ANCA are found in your blood you may have angiitis or IFD depend upon the medical history of patient A negative test means no ANCA are present in your blood and cause of symptoms are different needs further examination.

Yes it is available in the delhi at much effective cost price you can choose best diagnosis centre depend upon their ranking and price range

No special preparation is required for this, only you have to give the blood sample.

Related Tests

Health Packages

Early check ups are always better than delayed ones. Safety, precaution & care is depicted from the several health checkups. Here, we present simple & comprehensive health packages for any kind of testing to ensure the early prescribed treatment to safeguard your health.

view all health packages