Acute Myeloid Leukemia (AML) Characterization and Karyotyping Test Cost & Procedure

Your doctor will order blood tests and look for physical indications of acute myeloid leukemia (AML) before making the diagnosis. The soft tissue that forms all blood cells is found in the middle of bones. White blood cells that would usually differentiate into cancerous cells do so. Acute refers to the disease's rapid growth and typically aggressive course. In the bone marrow of those who have AML, there are a lot of abnormally immature white blood cells. The cells replace healthy blood cells and multiply very quickly. They also have a higher risk of bleeding due to the decline in the number of healthy blood cells.

What does the NPM1 gene mutation test for acute myeloid leukemia entail?

Nucleophosmin (NPM1), a multifunctional nucleo-cytoplasmic shuttling protein that is mostly found in the nucleoli, is encoded by the NPM1 gene, which is located at chromosome 5q35. An increase in blasts in the blood and/or bone marrow is a symptom of acute myeloid leukemia (AML), a clonal illness of hematopoietic progenitor cells that also suppresses healthy hematopoiesis. With up to 55% of adult patients having nonrandom chromosomal abnormalities, which can typically be identified by traditional karyotyping, AML is increasingly being recognized as a diverse illness. Cytogenetically normal AML makes up 20 to 25% of juvenile AML cases, which is a lower incidence than in adults.

Preparation

• No extra planning is required. It is advised that you either wear a blouse with no sleeves, half sleeves, or full sleeves that can easily be pulled up to your elbows so that the doctor may collect a blood sample without difficulty.
• If a specimen needs to be stored before being shipped, do so between 2 and 8 degrees Celsius.

Uses:

• A better prognosis is assumed for patients who have an NPM1 mutation and a normal karyotype.
• Prognosis may also be impacted by additional genetic markers.

Procedure Sample to be gathered:

3 mL of buffered solutions without fixatives and heparin, EDTA, or ACD cell pellets in cell culture media.

1 ug of extracted DNA from the past

Different methods are used to collect the aforementioned sample.

The reports are obtained within 8 to 10 days.

Limitations of NPM1 gene mutations in acute myeloid leukemia Test :

The profile's probes do not target any molecular mutations or chromosomal changes that could be found