ALL FISH Comprehensive Panel with Karyotyping Test Cost & Procedure

Abstract

The molecular cytogenetic examination of metaphase preparations using spectral microscopy is known as spectral karyotyping (SKY), a new fluorescence in situ hybridization (FISH) technique. 24 chromosome-specific painting probes are utilized in a single FISH assay to study the Development of human metaphase chromosomes. Deteriorate oligonucleotide-primed PCR is utilized to mark the investigations utilizing three fluorochromes and two haptens.

Every chromosome has a distinct spectral signature as a result of each probe being variably tagged with one, two, three, or four fluorescent dyes. Next in situ hybridization and immunodetection, a spectral impression is obtained utilizing a conventional fluorescent light microscope dressed with a specially developed triple-bandpass filter and the SpectraCube, which can compile spectral data for each pixel in a digital CCD portrait. Chromosome classification and the 24-color display are based on the distinctive emission spectra of the individual chromosomes. A A complicated examination of chromosomal irregularities is furnished, concurrently with data on chromosome banding from an inverted DAPI or a G-banded metaphase.

Acute Lymphoblastic Leukemia FISH Panels

A leukemia of immature B- or T-lineage lymphoid cells, acute lymphoblastic leukemia (ALL) is aggressive. B-cell ALL (B-ALL) primarily affects young children. When youngsters and grown-ups with B-ALL are foremost analyzed, hazard category and antidote options are established on the designation of gene rearrangements utilizing FISH testing.

Disease Overview

Incidence

The most prevalent leukemia in children is B-ALL, which affects 1.6/100,000 people annually

Symptoms

• Constitutional symptoms (such as fever, lethargy, and weight loss) and bone marrow failure (such as anemia, thrombocytopenia, and leukopenia) are frequent.
• Pain in the extremities or joints may be the only symptom observed in youngsters.

Test Interpretation

Test Results

FISH for children

Normal: no indication of copy number gain with CEP4 and/or CEP10, ETV6-RUNX1, BCR-ABL1 , KMT2A (MLL) rearrangement, or RUNX1 amplification.

Unusual: one of the aforementioned rearrangements or translocations was discovered

mature FISH

Normal: no signs of the BCR-ABL1, KMT2A (MLL), TCF3 (E2A), IGH, or MYC rearrangements.

Any of the aforementioned rearrangements, translocations, or copy number changes are abnormal.

ALL FISH PH-Like

Normal: no indication of CRLF2, JAK2, EPOR, CSF1R, ABL1, ABL2, or PDGFRB rearrangement

One of the mentioned rearrangements was found to be abnormal.

Forecasting Problems

The ARUP Consult Acute Lymphoblastic Leukemia topic has more details on the prognostic importance of discovered genomic rearrangements.

Limitations

Panels only identify the precise aberrations that the supplied FISH probes are designed to detect. Chromosome changes outside of the complimentary areas to these probes won't be found.