AFB-Identification & Speciation MTB and MOTT by Hains Test Cost & Procedure

Description

This test is designed to identify organisms that are partially acid fast or acid fast bacilli (AFB). The elevated grade of determination is fetched out, which is often to the species grade but may occasionally be to the subspecies level for some organisms (like Mycobacterium abscessus subsp. abscessus). The various platforms, assays, and methods that can be used are listed below.

When sending pure isolates to the lab for identification, this test is required (e.g. AFID). For clinical samples that required organism identification, it does not need to be ordered separately because those tests already include it (e.g. AFB1 and AFB3)

Abstract

Mycobacterium other than tuberculosis (MOTT), a fast-growing cultured acid-fast bacillus, is evaluated for susceptibility to drugs in AFB Drug susceptibility (MOTT). This test is helpful in determining whether the Acid-fast bacillus is present in blood.

Acid-fast bacteria, commonly known as acid-fast bacilli, are denoted by the acronym AFB. These bacteria belong to a group that has the trait of acid fastness, or the capacity to physically withstand acid-induced colour decolorization during staining operations. This distinctive quality aids in the classification and identification of acid-fast bacteria like Mycobacteria.

Because the microorganism is developing antibiotic resistance, susceptibility testing is essential. The AFB drug susceptibility test evaluates how sensitive an AFB culture is to various classes of antibiotics in order to identify whether the bacteria are susceptible to them or not.

Collect

mycobacterial isolates or organisms that are somewhat acid fast in solid media such as broth (7H9, MGIT, MP BACT/Alert, VersaTrek), Middlebrook slant (7H10 or 7H11), or Lowenstein-Jensen medium slant.

Volume of the specimen: The lab must receive an isolate with evident growth on solid media or an isolate in broth media (3 mL).

Intolerable Situations

cultures that were sent with faulty identities.

cultures mixed; take note that efforts will be made to isolate the organisms before rejection. In these circumstances, a reisolation fee will be assessed.mold-filled cultures.

Insufficient growth on solid media.

liquid medium containing 2 mL.

specimens that leak

Storage Temp of Transport Mail or transport isolates at room temperature. IATA/DOT category B requirements for non-tuberculous mycobacteria (NTM) and category A requirements for isolates of the M. tuberculosis complex should be followed when shipping biological materials.

Analysis of the Data

Where possible or suitable, species or subspecies levels of identification are used for organisms growing in pure cultures.