Acid-fast bacillus (AFB) Sensitivity-10 Drugs Test Cost & Procedure

This test is used to diagnose tuberculosis (TB), detect and identify mycobacterial infections, and track the efficacy of treatment. The gold standard for diagnosing infections is culture. Mycobacterium speciation is included in this test. Unlike conventional culture, which takes 4-6 weeks, rapid automated culture enables early recovery of mycobacteria within 7 days.

Acid-fast bacteria also understood as acid-fast bacilli are known as AFBs. These bacteria belong to a group that has the trait of acid fastness, or the capacity to physically withstand acid-induced color decolorization during staining operations. This distinctive quality aids in the classification and identification of acid-fast bacteria like Mycobacteria.

One of the diseases with a high mortality rate is tuberculosis (TB), which is brought on by the bacteria Mycobacterium tuberculosis (MTB). Because the microorganism is developing antibiotic resistance, susceptibility testing is essential. The AFB drug susceptibility test evaluates how sensitive an AFB culture is to various classes of antibiotics to identify whether the bacteria are susceptible to them or not.

The first-line TB medications Streptomycin, Isoniazid, Rifampicin, Ethambutol, and Pyrazinamide are assessed for their ability to inhibit the acid-fast bacilli (Mycobacterium tuberculosis) in the AFB Drug Susceptibility (SIREP) 1st line test.

Tests for Drug Susceptibility

Drug susceptibility testing is a challenging method to standardize, and competence in carrying out these tests necessitates knowledge of numerous components, including the

the causes of medication resistance and the indicators of it

When manipulated in a lab, medications' potency and stability

Drugs' antimycobacterial properties when put into various mediums

Results reading, interpretation, and reportingThis technique enables quantification of the percentage of drug-resistant mutants and can identify the 1% threshold at which therapeutic success is likely to be unsuccessful.

The following medicines are used in this method to test M. tuberculosis complex:

• Isoniazid
• Rifampin
• Ethambutol
• Ciprofloxacin
• Ofloxacin
• Streptomycin
• Kanamycin
• Capreomycin
• Amikacin
• RifabutinsEthionamide

The MGIT 960 method is used to measure the pyrpyrazinamide para-aminosalicylic acid (PAS).