Stain AFB (Kinyoun) Test Cost & Procedure

Joseph J. Kinyoun is the inventor of the Kinyoun method, commonly referred to as the Kinyoun stain (cold method). It is a differential staining method utilized to distinguish acid-fast bacteria-fast and bacteria that are not. The apicomplexan genus Cryptosporidium and other acid-fast bacteria including Mycobacterium and Nocardia are detected with this staining technique.

The Kinyoun-Cold Method's goal

Acid-fast classification Parasites like Cryptosporidium and Isopora spp. as like Mycobacterium spp.

Principle of the kinyoun-cold method

Mycolic acid, which exists in the outer membrane of acid-fast mycobacteria, creates the cells waxy and resistant to dyeing aqueous-based dyes, for example the Gram stain. Carbolfuchsin is employed as the principal stain and phenutilizedilised to help it penetrate the waxy surface of acid-fast bacteria. 1% sulfuric acid is used to cure the discoloration and eliminate any surplus. The cells are thereafter dyed with methylene blue, a secondary stain.

Procedure

• the method due to Kinyoun-Cold Before is to stain, prepare and fix the specimen smear.
• Carbolfuchsin should be used in the smear for 3 to 5 minutes at chamber temperature.
• Gently run water over the slide.
• Run the slide under 1% sulfuric acid decolorizer for nearly three minutes.
• After totally cleaning it with water, decolorize the slide once more for 1–2 minutes, or until the solution is transparent.
• Utilize the water to wash the slide.
• For one minute, mask the smear with the methylene blue secondary or counterstain.
• Gently run water over the slip.
• With some tissue paper, blot the slide to dry

Interpretation of Kinyoun-Cold Method

Pinkish organisms, for example, bacteriuria spp., are acid-fast.

Note: One acid-fast bacillus found in a single sputum sample is regarded as diagnostic. concolorous of nonacid-fast organisms is blue. The background material should also be stained blue.

Limitations there the  of kinyoun-cold method

1. It might be less accurate than the Ziehl-Neelsen technique.

2. Too-thick smears might not stain properly.