Double-Stranded DNA (dsDNA) Antibody Crithidia, IFA Test Cost & Procedure

This test is performed to determine whether anti-dsDNA IgG antibodies are present and is known as IFA titre (Crithidia luciliae indirect fluorescent test [CLIFT]). Systemic lupus erythematosus patients are expressly encouraged to use CLIFT (SLE). Most individuals with inactive SLE may have positive ELISA results for anti-dsDNA IgG but negative results for CLIFT.

Systemic lupus erythematosus (SLE): What Is It?

One of the deadly autoimmune illnesses is systemic lupus erythematosus (SLE). The body reacts to autoimmune diseases by attacking the healthy cells in the organs and tissues. The kidneys, brain, joints, skin, and other organs are all impacted.

SLE causes:

It is unknown what causes SLE the most frequently. Any of the causes mentioned above could be the cause of the illness:

• GeneticsEnvironmentalsHormonal certain medications
• Epidemiology
• Women are more likely to have SLE. At any age, it might happen.
• African Americans, Asian Americans, African Caribbeans, and Hispanic Americans are more likely to develop the illness.

The following are SLE patient symptoms:

• Arthritis may occur if there is joint discomfort and swelling.
• TJointsnts of the fingers, hands, wrists, and knees most frequently.
• Chest ache after inhaling deeply.
• Fatigue.
• A fever that has no known etiology.

• general unease, discomfort, or unwell feeling
• hair fall
• Loss of weight
• oral sores
• sunlight sensitivity
• Butterfly rash on the skin
• pains and weakness
• convulsions with tingling numbness
• vision issues
• changes in personality and memory

What is an anti-dsDNA antibody?

For ANA, there are two techniques:

IFA, or indirect fluorescent antibody In this technique, cells on a slide are combined with a patient's blood sample. The blood-borne autoantibodies interact with the cells. Under a microscope, the fall is mixed with a fluorescent antibody reagent. Fluorescence's existence and pattern are noticed.

When is it advised to have this test?

It is suggested if you have positive anti-ds DNA (ANA) test findings and SLE symptoms include chronic fatigue, joint discomfort, and a butterfly-shaped rash on the cheeks and nose.

measuring blood anti-dsDNA antibodies

Special Instructions:

Tests are run using a straightforward blood sample.

There is no need for additional planning.

It will be beneficial if you let your doctor know about all of the pharmaceuticals, vitamins, dietary supplements, herbal or ayurvedic remedies, and homeopathic medications you are using.

Results interpretation:

If anti-dsDNA antibody titres are dangerous (10 IU/ml),

inconclusive (5–9 IU/ml),

or positive (>10 IU/ml).