What is Mole?
Moles are different types of structures on skin of human, which are characterized by different colors ranging from black to dark brown and dark red, with irregular shape, different sizes and having various elevations.
They may be benign or malignant. The most common feature of malignant mole is its speedy increasing size within small time duration, with the further discussed symptoms, and should be consulted with doctor as soon as possible.
BRAF, the Most Possible Reason Behind Malignant Mole: Its Overview
BRAF & its Location
BRAF gene is a type of proto-oncogene which is present as growing factor for cells, inside the human body. It is located in the chromosome no. 7 of human cell nucleus.
What is the Actual Function of Braf in Normal Human Body?
BRAF gene is responsible for the division, maturation and stabilization of matured cells inside the human body, by encoding a protein commonly called B-Raf, and scientifically, referred to as Serine/Threonine-protein Kinase B-Raf. This B-Raf protein engages in the signaling pathways of cell growth.
Mutation of Braf and Its Effects on Human Body
BRAF proto-oncogene acts as the guide book for the production of BRAF protein, and decides the action, work and fate of the BRAF protein. So, any kind of change or mutation in the sequential base pair of BRAF gene, changes the whole function of BRAF protein.
Due to mutation in the sequence of BRAF gene, BRAF protein become hyperactive and causes uncontrollable cell growth, generally leads to the formation of malignant tumors.
Mutated BRAF gene converts itself from being Proto-oncogene to Oncogene, and causes defective growth of B-Raf protein and thus oncogenic tumor growth happens.
Site of Mutation in Braf Gene
Till date, BRAF mutations have been in fore-front of cancer research based upon mutation. BRAF has various kinds of mutations, among which BRAF V600E mutation has caught the eye of researchers as the most available.
What Does Braf V600e Stands For?
B-Raf, V standing for Valine and E standing for Glutamic acid, where, 600 means the exact number of amino acid (amino-acid 600), where the mutation took place.
What Are the Disorders or Diseases Caused by the Mutation of Braf V600e?
- Melanoma: Skin cancer
- Hairy Cell Leukemia
- Ovarian Cancer
- Thyroid Cancer
- Some specific types of Brain Cancers
- Colon cancer or Colorectal Cancer
When Should You Consult Your Physician?
If you are facing prolonged symptoms like the following, you should definitely go for diagnosis process and medical consultation.
- Moles of various shapes, elevated, differently coloured, sizes, evolving in size (In case of Melanoma)
- Fatigue (Common)
- Fever (Common)
- Appetite loss (Common)
- Night sweats, sleeplessness (Common)
- Prone to infection (Common)
- Lump and swollen lymph nodes on neck, difficulty in swallowing (Thyroid cancer)
- Imbalance in digestion and pain in colon, with Diarrhoea or constipation (Colorectal cancer)
Treatment
There have been different types of BRAF inhibitors discovered by the researchers which can be used as treatment for these BRAF mutation-caused diseases. Otherwise, chemotherapy, and radiations are preferred for patients already suffering from carcinoma caused by BRAF mutation.
For Free Consultation from the Doctor
- Contact- Dr. Sonal Sharma,(MBBS, MD in Pathology)
- Available: 24×7×365
- Phone Number: +919212125996
Diagnosis of BRAF V600E mutation in human body
BRAF V600E mutation results in the alteration of produced protein B-Raf, and thus causing Carcinogenic disorders. BRAF V600E defect or mutation is possible by Real Time or Quantitative Polymerase Chain Reaction (PCR).
Sample collection
The Sample collected could be from the various sources, such as:
- Tumor Tissue
- Bone Marrow
- Blood
What is Quantitative or Real Time-PCR?
Quantitative or Real Time-PCR is a highly accurate and sensitive molecular biology technique to detect BRAF V600E Mutated gene in sample, where we can detect any type of defect in the general standard gene sequence and the patient’s defective gene sequence.
Steps of Quantitative or Real Time-PCR
- Extraction: Sample bloods are treated with chemicals to have extracted or separated DNA from them.
- Performing Polymerase Chain Reaction:Denaturation, Annealing, Extension or Amplification of the targeted gene of BRAF V600E.
- Repetition of the cycle: Total number of cycle for the process will be 40.
- Time of report: The report of diagnosis with Quantitative -PCR technique is 72 hours.
Obtaining result: The sensitivity and reproducibility of result is optimized using standards and the obtained record of amplification of concerned gene BRAF V600E, with whatever changes in the genetic base or mutation detected in the V600E region, will be analyzed by Molecular Biologist, will be followed by final medical advisory by Doctor.
- Indication of Positive and Negative Result: Any kind of mutation in the concerned gene BRAF V600E and its amplification can be diagnosed as cause of cancer or malignancy of above-discussed part or organ of the body, depending upon the source of sample tested. Whereas, it will be considered negative for detection of no mutated gene sequence.
Quantitative Real Time-Pcr Test for Braf V600e Mutation Detection at Ganesh Diagnostic and Imaging Centre
Ganesh Diagnostic and Imaging Centre, a renowned and leading medical diagnostic laboratory in India, is having the best and highest accurate Quantitative Real Time-PCR machine, to diagnose genetic mutation leading to Carcinogenesisin the most reliable way, reasonable price, and less time. Book now!
Latest Quantitative Real Time-PCR machines used for diagnosis of Mutation in BRAF V600Eare listed here:
- Truelab Quattro Real-Time Quantitative PCR Machine
- CFX96 Bio-Rad Real-Time PCR Machine
- Genetix HT 96 Purifier Machine
- Genetix 32 purifier machine
- Biorad CFX96 Real Time System
- Truelab® Uno Dx Real Time Quantitative Micro Pcr Analyzer
Why to Choose Ganesh Diagnostic and Imaging Centre for Real Time Pcr Test to Diagnose Molecular Based Disorders?
- NABL, NABH accredited laboratory, Specialized in Molecular Oncology tests
- 24×365 open for patients of any kind of test, with availability of expert staffs
- Free home collection of all types of samples by Expert technicians and phlebotomists.
- Master Technicians at all departments
- Doctorate (Ph.D. & MD) Scientific officers and In-charges at all departments
- 50% Discount at kind of tests of all molecular biology tests
- Online availabilityof reports for all kinds of tests.
- Extra-caring and helpful staffs
- Ambulance availability for patients 24×365 free of cost
- Free transport facility for patients who come from distant locations
Visit Ganesh Diagnostic and Imaging Centre, Pathology Lab Near Me!
It is spread across the Delhi-NCR region to cater to your need.
